Enhanced chemiluminescence biosensor for the determination of phenolic compounds and hydrogen peroxide
نویسنده
چکیده
The enhanced chemiluminescence (CL) reaction of the luminol±H2O2±horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol±gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obtained for p-iodophenol, p-coumaric acid and 2-naphthol were 0.83 mM, 15 and 48 nM, respectively. Direct enzyme immobilization onto the end of the optical ®bre permits the construction of a remote enhanced CL biosensor. This remote biosensor has been applied to hydrogen peroxide assay (detection limit 52.2 mM and relative standard deviation 4.7% for n 4) and the results have been compared with the biosensor developed in cuvette. # 2001 Elsevier Science B.V. All rights reserved.
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